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human esc derived rgc  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc human esc derived rgc
    In vitro testing of R-28 cell-derived EVs on <t>RGC</t> survival and regeneration. Representative images of untreated control wells (A), R-28 cell-derived EVs treated wells (B), and CNTF-treated wells (C) are shown with the graphs showing the total surviving RGC number (D), the number of RGC with neurites (E), and the longest neurite length (F) in primary retinal cell culture after 3 days. Data are expressed as the mean ± SEM. Images were stained with a nuclear (DAPI, blue) and RGC marker (β-III tubulin, green). Scale bars: 50 µm. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; DAPI: 4′,6-diamidino-2-phenylindole; EV: extracellular vesicles; RGC: <t>retinal</t> <t>ganglion</t> <t>cells.</t>
    Human Esc Derived Rgc, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 96/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human esc derived rgc/product/WiCell Research Institute Inc
    Average 96 stars, based on 176 article reviews
    human esc derived rgc - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma"

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-24-00709

    In vitro testing of R-28 cell-derived EVs on RGC survival and regeneration. Representative images of untreated control wells (A), R-28 cell-derived EVs treated wells (B), and CNTF-treated wells (C) are shown with the graphs showing the total surviving RGC number (D), the number of RGC with neurites (E), and the longest neurite length (F) in primary retinal cell culture after 3 days. Data are expressed as the mean ± SEM. Images were stained with a nuclear (DAPI, blue) and RGC marker (β-III tubulin, green). Scale bars: 50 µm. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; DAPI: 4′,6-diamidino-2-phenylindole; EV: extracellular vesicles; RGC: retinal ganglion cells.
    Figure Legend Snippet: In vitro testing of R-28 cell-derived EVs on RGC survival and regeneration. Representative images of untreated control wells (A), R-28 cell-derived EVs treated wells (B), and CNTF-treated wells (C) are shown with the graphs showing the total surviving RGC number (D), the number of RGC with neurites (E), and the longest neurite length (F) in primary retinal cell culture after 3 days. Data are expressed as the mean ± SEM. Images were stained with a nuclear (DAPI, blue) and RGC marker (β-III tubulin, green). Scale bars: 50 µm. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; DAPI: 4′,6-diamidino-2-phenylindole; EV: extracellular vesicles; RGC: retinal ganglion cells.

    Techniques Used: In Vitro, Derivative Assay, Control, Cell Culture, Staining, Marker

    R-28 cell-derived EVs promote human ESC-derived RGC survival in vitro . Images show untreated controls, R-28 cell-derived EV treated, and CNTF-treated hESC-derived RGCs (green, βIII-tubulin) after injury induced by the microtubule poison, colchicine. Scale bar: 50 µm. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; ESC: embryonic stem cells; EV: extracellular vesicles; RGC: retinal ganglion cells.
    Figure Legend Snippet: R-28 cell-derived EVs promote human ESC-derived RGC survival in vitro . Images show untreated controls, R-28 cell-derived EV treated, and CNTF-treated hESC-derived RGCs (green, βIII-tubulin) after injury induced by the microtubule poison, colchicine. Scale bar: 50 µm. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; ESC: embryonic stem cells; EV: extracellular vesicles; RGC: retinal ganglion cells.

    Techniques Used: Derivative Assay, In Vitro

    R-28 cell-derived EVs show protective trend for RGCs in a chronic glaucoma model. (A) Experimental design of the in vivo study. R-28 cell-derived EVs were intravitreally injected weekly beginning 1 week after microbead injection, and animals’ IOPs were measured twice a week. Four weeks after weekly EV injection, animals were sacrificed and histologically analyzed. After injection, microbeads localized (arrow) around the iridocorneal angle (B). IOP (mmHg) of healthy animals (blue) and animals receiving intracameral injection of microbeads with (green) or without (brown) intravitreal EV treatments is shown (C). (D, E) Representative images (D) and quantification (E) of Brn3a + (green) RGCs from the three groups on week 5. Scale bars: 50 µm. Data are presented as mean ± SEM. n = 3–5. EV: Extracellular vesicles; IOP: intraocular pressure; RGC: retinal ganglion cells; PBS: phosphate buffered saline.
    Figure Legend Snippet: R-28 cell-derived EVs show protective trend for RGCs in a chronic glaucoma model. (A) Experimental design of the in vivo study. R-28 cell-derived EVs were intravitreally injected weekly beginning 1 week after microbead injection, and animals’ IOPs were measured twice a week. Four weeks after weekly EV injection, animals were sacrificed and histologically analyzed. After injection, microbeads localized (arrow) around the iridocorneal angle (B). IOP (mmHg) of healthy animals (blue) and animals receiving intracameral injection of microbeads with (green) or without (brown) intravitreal EV treatments is shown (C). (D, E) Representative images (D) and quantification (E) of Brn3a + (green) RGCs from the three groups on week 5. Scale bars: 50 µm. Data are presented as mean ± SEM. n = 3–5. EV: Extracellular vesicles; IOP: intraocular pressure; RGC: retinal ganglion cells; PBS: phosphate buffered saline.

    Techniques Used: Derivative Assay, In Vivo, Injection, Saline

    Differentially expressed miRNA shown as abundance and fold change heat map profiles. Heatmaps show the upregulated and downregulated normalized counts of miRNA from injured RGCs treated with R-28 cell-derived EVs compared to injured untreated (A, B), injured RGCs treated with R-28 derived EVs compared to uninjured treated (D, E), and uninjured RGCs treated with R-28 cell-derived EVs compared to injured untreated (G, H), both statistically significant ( P < 0.05; A, D, G) and those trending towards significance ( P < 0.1; B, E, H) with abundance profiles shown in associated bar charts (C, F, I, respectively). * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 3. EV: Extracellular vesicles; RGC: retinal ganglion cells.
    Figure Legend Snippet: Differentially expressed miRNA shown as abundance and fold change heat map profiles. Heatmaps show the upregulated and downregulated normalized counts of miRNA from injured RGCs treated with R-28 cell-derived EVs compared to injured untreated (A, B), injured RGCs treated with R-28 derived EVs compared to uninjured treated (D, E), and uninjured RGCs treated with R-28 cell-derived EVs compared to injured untreated (G, H), both statistically significant ( P < 0.05; A, D, G) and those trending towards significance ( P < 0.1; B, E, H) with abundance profiles shown in associated bar charts (C, F, I, respectively). * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 3. EV: Extracellular vesicles; RGC: retinal ganglion cells.

    Techniques Used: Derivative Assay



    Similar Products

    human esc derived rgc  (WiCell Research Institute Inc)
    96
    WiCell Research Institute Inc human esc derived rgc
    In vitro testing of R-28 cell-derived EVs on <t>RGC</t> survival and regeneration. Representative images of untreated control wells (A), R-28 cell-derived EVs treated wells (B), and CNTF-treated wells (C) are shown with the graphs showing the total surviving RGC number (D), the number of RGC with neurites (E), and the longest neurite length (F) in primary retinal cell culture after 3 days. Data are expressed as the mean ± SEM. Images were stained with a nuclear (DAPI, blue) and RGC marker (β-III tubulin, green). Scale bars: 50 µm. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; DAPI: 4′,6-diamidino-2-phenylindole; EV: extracellular vesicles; RGC: <t>retinal</t> <t>ganglion</t> <t>cells.</t>
    Human Esc Derived Rgc, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human esc derived rgc/product/WiCell Research Institute Inc
    Average 96 stars, based on 1 article reviews
    human esc derived rgc - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    In vitro testing of R-28 cell-derived EVs on RGC survival and regeneration. Representative images of untreated control wells (A), R-28 cell-derived EVs treated wells (B), and CNTF-treated wells (C) are shown with the graphs showing the total surviving RGC number (D), the number of RGC with neurites (E), and the longest neurite length (F) in primary retinal cell culture after 3 days. Data are expressed as the mean ± SEM. Images were stained with a nuclear (DAPI, blue) and RGC marker (β-III tubulin, green). Scale bars: 50 µm. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; DAPI: 4′,6-diamidino-2-phenylindole; EV: extracellular vesicles; RGC: retinal ganglion cells.

    Journal: Neural Regeneration Research

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    doi: 10.4103/NRR.NRR-D-24-00709

    Figure Lengend Snippet: In vitro testing of R-28 cell-derived EVs on RGC survival and regeneration. Representative images of untreated control wells (A), R-28 cell-derived EVs treated wells (B), and CNTF-treated wells (C) are shown with the graphs showing the total surviving RGC number (D), the number of RGC with neurites (E), and the longest neurite length (F) in primary retinal cell culture after 3 days. Data are expressed as the mean ± SEM. Images were stained with a nuclear (DAPI, blue) and RGC marker (β-III tubulin, green). Scale bars: 50 µm. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; DAPI: 4′,6-diamidino-2-phenylindole; EV: extracellular vesicles; RGC: retinal ganglion cells.

    Article Snippet: To test the therapeutic effect of R-28-derived EVs on human ESC-derived RGC (H7/H9 immortalized cell line; WiCell, Madison, WI, USA, #WA07, RRID: CVCL_S800) were differentiated from CRISPR-modified ESC generously donated from Prof Donald Zacks laboratory (Johns Hopkins University, Baltimore, MD, USA) and licensed for use from WiCell (Material Transfer Agreement issue-164634007).

    Techniques: In Vitro, Derivative Assay, Control, Cell Culture, Staining, Marker

    R-28 cell-derived EVs promote human ESC-derived RGC survival in vitro . Images show untreated controls, R-28 cell-derived EV treated, and CNTF-treated hESC-derived RGCs (green, βIII-tubulin) after injury induced by the microtubule poison, colchicine. Scale bar: 50 µm. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; ESC: embryonic stem cells; EV: extracellular vesicles; RGC: retinal ganglion cells.

    Journal: Neural Regeneration Research

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    doi: 10.4103/NRR.NRR-D-24-00709

    Figure Lengend Snippet: R-28 cell-derived EVs promote human ESC-derived RGC survival in vitro . Images show untreated controls, R-28 cell-derived EV treated, and CNTF-treated hESC-derived RGCs (green, βIII-tubulin) after injury induced by the microtubule poison, colchicine. Scale bar: 50 µm. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates. CNTF: Ciliary neurotrophic factor; ESC: embryonic stem cells; EV: extracellular vesicles; RGC: retinal ganglion cells.

    Article Snippet: To test the therapeutic effect of R-28-derived EVs on human ESC-derived RGC (H7/H9 immortalized cell line; WiCell, Madison, WI, USA, #WA07, RRID: CVCL_S800) were differentiated from CRISPR-modified ESC generously donated from Prof Donald Zacks laboratory (Johns Hopkins University, Baltimore, MD, USA) and licensed for use from WiCell (Material Transfer Agreement issue-164634007).

    Techniques: Derivative Assay, In Vitro

    R-28 cell-derived EVs show protective trend for RGCs in a chronic glaucoma model. (A) Experimental design of the in vivo study. R-28 cell-derived EVs were intravitreally injected weekly beginning 1 week after microbead injection, and animals’ IOPs were measured twice a week. Four weeks after weekly EV injection, animals were sacrificed and histologically analyzed. After injection, microbeads localized (arrow) around the iridocorneal angle (B). IOP (mmHg) of healthy animals (blue) and animals receiving intracameral injection of microbeads with (green) or without (brown) intravitreal EV treatments is shown (C). (D, E) Representative images (D) and quantification (E) of Brn3a + (green) RGCs from the three groups on week 5. Scale bars: 50 µm. Data are presented as mean ± SEM. n = 3–5. EV: Extracellular vesicles; IOP: intraocular pressure; RGC: retinal ganglion cells; PBS: phosphate buffered saline.

    Journal: Neural Regeneration Research

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    doi: 10.4103/NRR.NRR-D-24-00709

    Figure Lengend Snippet: R-28 cell-derived EVs show protective trend for RGCs in a chronic glaucoma model. (A) Experimental design of the in vivo study. R-28 cell-derived EVs were intravitreally injected weekly beginning 1 week after microbead injection, and animals’ IOPs were measured twice a week. Four weeks after weekly EV injection, animals were sacrificed and histologically analyzed. After injection, microbeads localized (arrow) around the iridocorneal angle (B). IOP (mmHg) of healthy animals (blue) and animals receiving intracameral injection of microbeads with (green) or without (brown) intravitreal EV treatments is shown (C). (D, E) Representative images (D) and quantification (E) of Brn3a + (green) RGCs from the three groups on week 5. Scale bars: 50 µm. Data are presented as mean ± SEM. n = 3–5. EV: Extracellular vesicles; IOP: intraocular pressure; RGC: retinal ganglion cells; PBS: phosphate buffered saline.

    Article Snippet: To test the therapeutic effect of R-28-derived EVs on human ESC-derived RGC (H7/H9 immortalized cell line; WiCell, Madison, WI, USA, #WA07, RRID: CVCL_S800) were differentiated from CRISPR-modified ESC generously donated from Prof Donald Zacks laboratory (Johns Hopkins University, Baltimore, MD, USA) and licensed for use from WiCell (Material Transfer Agreement issue-164634007).

    Techniques: Derivative Assay, In Vivo, Injection, Saline

    Differentially expressed miRNA shown as abundance and fold change heat map profiles. Heatmaps show the upregulated and downregulated normalized counts of miRNA from injured RGCs treated with R-28 cell-derived EVs compared to injured untreated (A, B), injured RGCs treated with R-28 derived EVs compared to uninjured treated (D, E), and uninjured RGCs treated with R-28 cell-derived EVs compared to injured untreated (G, H), both statistically significant ( P < 0.05; A, D, G) and those trending towards significance ( P < 0.1; B, E, H) with abundance profiles shown in associated bar charts (C, F, I, respectively). * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 3. EV: Extracellular vesicles; RGC: retinal ganglion cells.

    Journal: Neural Regeneration Research

    Article Title: R-28 cell-derived extracellular vesicles protect retinal ganglion cells in glaucoma

    doi: 10.4103/NRR.NRR-D-24-00709

    Figure Lengend Snippet: Differentially expressed miRNA shown as abundance and fold change heat map profiles. Heatmaps show the upregulated and downregulated normalized counts of miRNA from injured RGCs treated with R-28 cell-derived EVs compared to injured untreated (A, B), injured RGCs treated with R-28 derived EVs compared to uninjured treated (D, E), and uninjured RGCs treated with R-28 cell-derived EVs compared to injured untreated (G, H), both statistically significant ( P < 0.05; A, D, G) and those trending towards significance ( P < 0.1; B, E, H) with abundance profiles shown in associated bar charts (C, F, I, respectively). * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 3. EV: Extracellular vesicles; RGC: retinal ganglion cells.

    Article Snippet: To test the therapeutic effect of R-28-derived EVs on human ESC-derived RGC (H7/H9 immortalized cell line; WiCell, Madison, WI, USA, #WA07, RRID: CVCL_S800) were differentiated from CRISPR-modified ESC generously donated from Prof Donald Zacks laboratory (Johns Hopkins University, Baltimore, MD, USA) and licensed for use from WiCell (Material Transfer Agreement issue-164634007).

    Techniques: Derivative Assay